Hs 936.T(C1)細(xì)胞
ATCC Number： CRL-7687?
相關(guān)**： 黑色素瘤
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
器官來源： 皮膚
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
Hs 936.T(C1)細(xì)胞物種來源： 人
Designations: Hs 936.T(C1)
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Hs 936.T(C1)細(xì)胞Cellular Products: melanin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments: Clone of ATCC CRL-7686.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 936.T(C1)細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO 5%
Storage temperature:Hs 936.T(C1)細(xì)胞 liquid nitrogen vapor
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
