SW48細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
器官來(lái)源： 結(jié)腸
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
年限： Dukes' type C, grade IV
ATCC Number： CCL-231?
相關(guān)**： 大腸癌
SW48細(xì)胞Designations: SW48 [SW-48]
Depositors: A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes' type C, grade IV
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 0.6 ng/10 exp6 cells/10 days; keratin
Permits/Forms: SW48細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Tumorigenic: Yes
Antigen Expression: HLA A32, A33, B27, B35; blood type AB; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 9, 10
D13S317: 11, 12
D16S539: 11, 13
D5S818: 10, 14
D7S820: 9, 10
THO1: 6, 9.3
TPOX: 8
vWA: 18, 20, 21
SW48細(xì)胞Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Age: 82 years
Gender: female
Ethnicity: Caucasian
Comments: CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
Propagation: SW48細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol:
Culture never becomes 100% confluent. Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels..
Incubate cultures at 37?C.
Subcultivation Ratio: SW48細(xì)胞A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22140: . . In Vitro 8: 443, 1973.
22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615
22260: Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
25093: Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479
32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066
