JM1細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-10423?
相關(guān)**： 其他**
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
JM1細(xì)胞Designations: JM1
Depositors: Immunex Corporation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: B cell lymphoma
Cell Type: pre-B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. JM1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
DNA Profile (STR): D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Comments: JM1細(xì)胞The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.The cells are negative for CD7, My 7, My 9 and surface IgM.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: JM1細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 91916: Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717
