BJ-5ta細胞
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 人
器官來源： 其他
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
數(shù)量： 大量
年限： newborn
ATCC Number： CRL-4001?
細胞形態(tài)： 成纖維樣
Designations: BJ-5ta
Depositors: Geron Corporation
BJ-5ta細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin, foreskin
Cell Type: fibroblast immortalized with hTERT
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing.
Applications: BJ-5ta細胞TAs part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation.
We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when firstrecovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332].
Reverse Transcript: N
Antigen Expression: fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.) (fibroblast surface protein (FSP) was assayed by flow cytometry.)
cytokeratin; Homo sapiens(cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody) (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,9
D16S539: 9,13
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 10,11
vWA: 16,18
Cytogenetic Analysis: BJ-5ta細胞This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal.
Age: newborn
Gender: male
HeLa Markers: N
Comments: The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332].
TAs part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when firstrecovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Propagation: ATCC complete growth medium: A 4:1 mixture of Dulbecco's medium and Medium 199 with supplements as follows :
4 parts of Dulbecco's Modified Eagle's Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate
1 part of Medium 199
Supplemented with:
0.01 mg/ml hygromycin B
10% fetal bovine serum
Atmosphere: air, 95%; BJ-5ta細胞carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture when cell concentration reaches between 8 X 10(3) and 1 X 10(4) cells/cm2.
Subculturing: Protocol: Volumes are given for a 75-cm2 flask; proportionally reduce or increase the amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Add 3.0 to 5.0 ml of 0.25% trypsin, 0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not hit or shake the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 10.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 3 x 10(3) to 5 x 10(3) viable cells/cm2 is recommended.
Subcultivation Ratio: 1:2 to 1:3 twice weekly
Medium Renewal: every 2 to 3 days
Preservation: Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 62 hr
Related Products: recommended serum:ATCC 30-2020
Trypsin-EDTA Solution:ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
plasmid in bacteria:ATCC MBA-141
References: 47354: BJ-5ta細胞Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332
90421: Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802
