1H11細胞

ATCC Number： HB-10611?

生長狀態： 懸浮生長

細胞形態： **樣

**類型： IgG1

運輸方式： 凍存運輸

數量： 大量

是否是腫瘤細胞： 0

1H11細胞物種來源： 小鼠

Designations: 1H11

Depositors: Washington Research Foundation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: 1H11細胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide

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Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.

Spleen cells were fused with mouse myeloma cells.

The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.

Propagation: 1H11細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22055: Eyre DR. 1H11細胞Hybridoma cell line producing an antibody to type-I collagen amino-terminal telopeptide. US Patent 5,300,434 dated Apr 5 1994