G28-5細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgG1
ATCC Number： HB-9110?
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: G28-5
G28-5細(xì)胞Depositors: Oncogen
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Bp50 (CD40, human B cell antigen)
Permits/Forms: G28-5細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with human tonsillar lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for the Bp50 (CD40) B cell surface antigen.
The antibody reacts specifically with CD20 positive (Bp35, B1) blood and tonsillar B cells, and does not react with T cells or NK cells.
It does react with malignant B cells and B cell lines.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: G28-5細(xì)胞Protocol: Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifigugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Maintain between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22015: Ledbetter JA, Clark EA. G28-5細(xì)胞Ligands and methods for augmenting B-cell proliferation. US Patent 5,182,368 dated Jan 26 1993
