129A3/1細(xì)胞
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
**類型： IgA
ATCC Number： HB-8087?
Designations: 129A3/1
Depositors: Johns Hopkins University
Isotype: IgA
129A3/1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 129A3/1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
129A3/1細(xì)胞Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. 129A3/1細(xì)胞US Patent 4,416,866 dated Nov 22 1983
