13.90.2細胞

運輸方式： 凍存運輸

生長狀態： 懸浮生長

數量： 大量

細胞形態： **樣

**類型： IgG1

ATCC Number： HB-8337?

是否是腫瘤細胞： 0

物種來源： 小鼠

13.90.2細胞Designations: 13.90.2

Depositors: American Cyanamid Co.

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 13.90.2細胞immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites and merozoites

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Tumorigenic: Yes

Comments: Animals were immunized with E. tenella merozoites.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with both merozoites and sporozoites by radioimmunoassay and by immunofluorescence.

The antibody cross reacts with sporozoites of E. necatrix, and E. maxima.

Propagation: 13.90.2細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

Recommended medium 13.90.2細胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 3597: Thammana P, et al. Antigens and monoclonal antibodies reactive against merozoites of Eimeria spp.. US Patent 4,650,676 dated Mar 17 1987